Concentration
While measuring concentrations of purified proteins is well-established, the same experiment conducted in serum or lysates containing mixtures of biomolecules represents a considerable challenge. MDS provides a straightforward protocol and analysis workflow to determine concentrations in any background matrix.
Overview
Existing antibody detection and biomarker assessment methods in serum, as well as overexpressed target protein detection in cell-culture supernatant or lysates, are qualitative, cumbersome, inaccurate, and prone to artifacts. Our MDS approach enables direct and accurate detection of molecules in complex mixtures without the need for standards. It provides universally comparable absolute molar concentrations, making interpretation easy. We offer a comprehensive workflow for various systems, covering sample preparation, data acquisition, and analysis.
In-solution determination of concentration.
Direct ELISA
- Challenging targets difficult to attach to surface
- Non-specific binding from complex sample backgrounds to surface
- Target avidity
Competition ELISA
- Measurement under native conditions with fluorescently tagged ligands
- No non-specific binding from complex sample backgrounds
Case Study
Microfluidic characterization reveals broad range of SARS-CoV-2 antibody affinity in human plasma.
Schneider et al., Life Science Alliance, vol 5 (2) Nov 2021. DOI: 10.25508/lsa.202101270.
Get started
To study the active concentration of biomolecular species in their native state in solution we recommend the following:
Workflow specification and benefits:
- 2-3 hours including sample equilibration.
- KD range from nM to µM
- Required volume of serum or plasma 60 µL
- Required amount of antigen 1 µg LLOQ for antibody concentration 10 nM
- Unique affinity-concentration profile
- Quick & easy to perform
